Wet-milling of buckwheat with hull and dehulled - The properties of the obtained starch fraction

The buckwheat with or without hull were used for the laboratory wet-milling. Starchy materials obtained was characterised by determining starch extraction efficiency, particle size distribution and microstructure. The pasting (RVA) and thermal properties (DSC) were also analysed. The starch extraction efficiency was higher for total starch isolated from buckwheat with hull compared to dehulled. The mean particle diameter of the pure starch isolated from both buckwheats was about 18 μm. The longer time of steeping of buckwheat with hull caused a decrease in temperature of gelatinization compared to the dehulled ones. Significantly higher enthalpy values for both pure starches compared to the other samples were noticed. The increasing of gelatinisation enthalpy with increasing of the steeping time and the higher protein amount was observed. The used wet-milling method did not change significantly the properties of the obtained starchy materials compared to raw material.The study was supported by Project REFRESH (FP7-REGPOT-2010-1-264105)-Unlocking the potential of the Institute of Animal Reproduction and Food Research for strengthening integration with the European Research Area and region development.Peer Reviewe

Myo-inositol hexakisphosphate degradation by Bifidobacterium pseudocatenulatum ATCC 27919 improves mineral availability of high fibre rye-wheat sour bread

The goal of this investigation was to develop baking products using Bifidobacterium pseudocatenulatum ATCC27919, a phytase producer, as a starter in sourdough for the production of whole rye-wheat mixed bread. This Bifidobacterium strain contributed to myo-inositol hexakisphosphate (phytate) hydrolysis, resulting in breads with higher mineral availability as was predicted by the phytate/mineral molar ratios, which remained below the inhibitory threshold values for Ca and Zn intestinal absorption. The products with sourdough showed similar technological quality as their homologous without sourdough, with levels of acetic and d/l lactic acids in dough and bread baking significantly higher with the use of sourdough. The overall acceptability scores showed that breads with 25% of whole rye flour were highly accepted regardless of the inclusion of sourdough. This work emphasises that the in situ production of phytase during fermentation by GRAS/QPS microorganisms constitutes a strategy which is particularly appropriate for reducing the phytate contents in products for human consumption.This work was financially supported by grants Consolider Fun-C-Food CSD2007-00063 and AGL2011-22669 from the Ministry of Economy and Competitiveness (MINECO) and PROMETEO/2012/064 from the Generalitat Valenciana, Spain.Peer Reviewe

Reduction of Phytate in Soy Drink by Fermentation with Lactobacillus casei Expressing Phytases From Bifidobacteria

Plant-based food products can be modified by fermentation to improve flavour and the concentration of some biologically active compounds, but also to increase the mineral availability by eliminating anti-nutrient substances such as phytates. The objective of this study was to develop a fermented soybean drink with improved nutritional quality and source of probiotic bacteria by including as starter for fermentation Lactobacillus casei strains modified to produce phytase enzymes from bifidobacteria. The L. casei strains showed a good adaptation to develop in the soy drink but they needed the addition of external carbohydrates to give rise to an efficient acidification. The strain expressing the Bifidobacterium pseudocatenulatum phytase was able to degrade more than 90 % phytate during product fermentation, whereas expression of Bifidobacterium longum spp. infantis phytase only led to 65 % hydrolysis. In both cases, accumulation of myo-inositol triphosphates was observed. In addition, the hydrolysis of phytate in soy drink fermented with the L. casei strain expressing the B. pseudocatenulatum phytase resulted in phytate/mineral ratios for Fe (0.35) and Zn (2.4), which were below the critical values for reduced mineral bioavailability in humans. This investigation showed the ability of modified L. casei to produce enzymes with technological relevance in the design of new functional foods.This work was financed by projects Consolider Fun-C-Food CSD2007-00063 from the Ministry of Economy and Competitiveness (MINECO) and PROMETEO/2012/064 from the Generalitat Valenciana, Spain.Peer Reviewe

Application of phytases from bifidobacteria in the development of cereal-based products with amaranth

The effects of the inclusion of purified phytases from Bifidobacterium longum spp. infantis ATCC15697 and Bifidobacterium pseudocatenulatum ATCC27919 on phytate (InsP6) levels were analyzed during breadmaking process. Two different levels of whole amaranth (Amaranthus cruentus) flour (25 and 50 %) were used in bread dough preparation, and they were compared to control doughs made with 100 % wheat flour and 100 % whole wheat flour. Bread samples made with 50 % of amaranth flour showed a significant decrease in technological quality parameters in comparison with control white breads. However, a 25 % of amaranth flour improved the nutritional value of the bread, with only a slight depreciation in the quality. Addition of bifidobacterial phytases resulted in higher InsP6 degradation compared with a commercial fungal phytase, without affecting the bread quality. InsP 6 reduction was especially efficient in breads with 25 % amaranth, leading to InsP6 levels below the threshold of mineral bioavailability inhibition for Fe and Zn in human nutrition.This work was financially supported by grants AGL2011-22669 and Consolider Fun-C-Food CSD2007-00063 from the Ministry of Economy and Competitiveness (MINECO) and PROMETEO/2012/064 from the Generalitat Valenciana, Spain. The contract of I. García-Mantrana from the Consolider Fun-C-Food Project is gratefully acknowledged.Peer Reviewe

Bread with whole quinoa flour and bifidobacterial phytases increases dietary mineral intake and bioavailability

The purpose of the present work was to provide further information on how replacing wheat flour by whole quinoa flour (at 25 and 50g/100g of flour) affects bread performance and to assess its potential as a nutritious ingredient. Bread with quinoa resulted in a depreciation in quality in terms of loaf specific volume (from 4.48 to 3.46/2.63 cm3/g), crumb firmness (from 0.77 to 1.55/2.64 N) and acceptability (from 7.94 to 7.58/5.94). Quinoa increased the bread nutritional value, raising fibre (from 5.5 to 7.2 g/100g) and minerals (Ca from 0.35 to 1.28 mg/g, Fe from 17 to 34 ¿g/g, and Zn from 23 to 48 ¿g/g). The phytates were controlled by bifidobacterial phytase treatment during breadmaking (from 4.7 ¿mol/g to below the detection limit), which decreased phytate/mineral molar ratios to values lower than the threshold for inhibition of Fe and Zn absorption. Quinoa could partially replace wheat flour in bread, increasing its nutritional value in terms of dietary fibre, minerals, proteins of high biological value and healthy fats, with only a small depreciation in bread quality at 25g/100g of flour substitution. The high phytate contents were efficiently removed by phytase treatment and the breads were accepted by consumers.This work was financially supported by grants Consolider Fun-C-Food CSD2007-00063 and AGL2011-22669 from the Ministry of Economy and Competitiveness (MINECO) and PROMETEO/2012/064 from the Generalitat Valenciana, Spain.Peer Reviewe

Evaluation of the technological, nutritional and sensory qualities of bakery products produced with partial substitution of the wheat flour by whole rice flour

El objetivo del presente trabajo fue determinar las características tecnológicas, nutricionales y sensoriales del pan elaborado por sustitución de 25 y 50% de la harina de trigo convencional por harina integral de arroz (Oryza sativa, L). Los productos desarrollados se evaluaron en términos de volumen específico del pan, aspecto de la rebanada central, dureza de la miga, contenido de humedad, fibra dietética, fitatos, minerales, cinética de retrogradación de la amilopectina por calorímetro diferencial de barrido y evaluación sensorial mediante escala hedónica de nueve puntos. En general, la incorporación de la harina integral de arroz a la formulación mostró diferencias significativas en cuanto a la cinética de retrogradación de la amilopectina en comparación con las muestras control (entalpía de retrogradación varió desde 2,4 a 1,8-2,1 J/g). También repercutió en una disminución del volumen de la pieza panaria (desde 4,48 a 2,26-2,86 cm3/g) que estuvo directamente relacionado con un aumento de la dureza de la miga (desde 0,77 a 1,90-3,85 N). No obstante, se observó una alta aceptación global por parte del consumidor (7,2-7,3). En cuanto a los minerales, el Ca y el Zn podrían estar biodisponibles en la formulación del 25% y en las muestras controles. En los productos con harina integral de arroz se observó un aumento significativo del nivel de trifosfatos de mio-inositol (desde valores despreciables a 0,38-0,44 μmoles/g), el cual se considera que ejerce actividad biológica positiva en el organismo. Es importante considerar que la biodisponibilidad de minerales en los productos integrales está relacionada con la formulación y el proceso de elaboración del pan, principalmente por la presencia de fitatos. Éstos están presentes en alta proporción en la formulación con 50% de harina integral de arroz, por lo que se estima una baja biodisponibilidad de minerales debido a que las relaciones molares fitato/mineral superan los valores de umbral de inhibición de la absorción de hierro y zinc.Este trabajo fue financiado por los proyectos Consolider Fun-C-Food CSD2007-00063 del Ministerio de Economía y Competitividad (MINECO) y PROMETEO/2012/064 de la Generalitat Valenciana, España.Peer reviewe

Expression of bifidobacterial phytases in Lactobacillus casei and their application in a food model of whole-grain sourdough bread

Phytases are enzymes capable of sequentially dephosphorylating phytic acid to products of lower chelating capacity and higher solubility, abolishing its inhibitory effect on intestinal mineral absorption. Genetic constructions were made for expressing two phytases from bifidobacteria in Lactobacillus casei under the control of a nisin-inducible promoter. L. casei was able of producing, exporting and anchoring to the cell wall the phytase of Bifidobacterium pseudocatenulatum. The phytase from Bifidobacterium longum spp. infantis was also produced, although at low levels. L. casei expressing any of these phytases completely degraded phytic acid (2 mM) to lower myo-inositol phosphates when grown in MRS medium. Owing to the general absence of phytase activity in lactobacilli and to the high phytate content of whole grains, the constructed L. casei strains were applied as starter in a bread making process using whole-grain flour. L. casei developed in sourdoughs by fermenting the existing carbohydrates giving place to an acidification. In this food model system the contribution of L. casei strains expressing phytases to phytate hydrolysis was low, and the phytate degradation was mainly produced by activation of the cereal endogenous phytase as a consequence of the drop in pH. This work shows the capacity of lactobacilli to be modified in order to produce enzymes with relevance in food technology processes. The ability of these strains in reducing the phytate content in fermented food products must be evaluated in further models.This work was financed by projects Consolider Fun-C-Food CSD2007-00063 from the Ministry of Economy and Competitiveness (MINECO) and PROMETEO/2012/064 from the Generalitat Valenciana, Spain.Peer reviewe

Development of Highly Nutritional Breads with By-Products of Chia (Salvia hispanica L.) Seeds

The effect of the incorporation of various types of residual chia flour (whole, semi-defatted and defatted, with or without mucilage) on the technological quality of bread was investigated. The various types of chia flour were used to substitute 5 and 10% wt/wt of wheat flour in the bread formulations. The water absorption, dough development time and stability of blends with the presence of mucilage and the incorporation of 10% wt/wt of chia flour demonstrated the highest values in comparison with the other ones. The specific volume of the flour variants with 5% wt/wt of chia flour with mucilage were similar to the control bread; while those formulated with chia flour without mucilage exhibited a lesser volume. The incorporation of 10% wt/wt of chia flour in the formulations caused a decrease in the technological quality of the bread as expected. The bread crust and crumb colour parameters were mainly influenced by the level of chia flour substitution, which resulted in a decrease in lightness and h values. The substitution of wheat flour with 5% wt/wt of chia flour counterparts with mucilage improved the technological quality of the breads. The different oil content of the chia flours did not show any significant influence on overall quality or texture.Fil: Guiotto, Estefania Nancy. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Consejo Superior de Investigaciones Científicas; EspañaFil: Tomás, Mabel Cristina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Haros, Claudia Monika. Consejo Superior de Investigaciones Científicas; Españ

Effect of whole amaranth flour on bread properties and nutritive value

This study investigated the effect of replacing wheat flour by whole. Amaranthus cruentus flour (up to 40 g/100 g) to evaluate its potential utility as a nutritious breadmaking ingredient. The incorporation of amaranth flour significantly increased protein, lipid, ash, dietary fibre and mineral contents. Breads with amaranth have significantly higher amounts of phytates and lower. myo-inositol phosphates, which could predict low mineral bioavailability at high levels of substitution (30-40 g/100 g). An increase in crumb hardness and elasticity was observed, and tristimulus colour values were significantly affected when the amaranth concentration was raised. Mineral contents, both micro- and macroelements, were increased significantly by the wheat flour substitution. Whole amaranth flour could be used as a partial replacement for wheat flour in bread formulations, increasing the product's nutritional value and raising dietary fibre, mineral and protein levels, with a significant slight depreciation in bread quality when used in proportions between 10 and 20 g/100 g. Thus, the inclusion of amaranth flour could be limited to a maximum proportion of 20 g/100 g, thereby maintaining both product quality as well as the nutritional benefit of this ingredient.This work was financially supported by projects AGL2006-09613/ALI, 200870I229, AGL2011-22669 and 2006PL0015 from the Ministry of Science and Innovation (MICINN), and PROMETEO/2012/064 from Generalitat Valenciana, Spain. The fellowship of J.M. Sanz Penella from MICINN is gratefully acknowledged.Peer Reviewe

Diet shapes the ability of human intestinal microbiota to degrade phytate - in vitro studies

Aims: Investigation of intestinal bacterial groups involved in phytate degradation and the impact of diets with different phytate contents on phytase activity. Methods and Results: Faecal samples of adults on conventional (n = 8) or vegetarian (n = 8) diets and breastfed infants (n = 6) were used as an inoculum for modified media supplemented with phytate. Populations of Gram-positive anaerobes (GPA), lactic acid bacteria (LAB), Proteobacteria-Bacteroides (P-B), coliforms and anaerobes were studied. The PCR-DGGE analysis revealed a random distribution of DGGE profiles in the dendrograms of GPA, P-B and coliforms, and a partially diet-specific distribution in the DGGE dendrograms of LAB and anaerobes. The degradation of phytic acid (PA) was determined with HPLC method in supernatants of the cultures. Regardless of the diet, the Gram-positive anaerobes and LAB displayed the lowest ability to degrade phytate, whereas the coliforms and P-B cultures produced higher amounts of intermediate myo-inositol phosphates. Bacterial populations grown in a nonselective medium were the most effective ones in phytate degradation. It was the vegetarians' microbiota that particularly degraded up to 100% phytate to myo-inositol phosphate products lower than InsP3. Conclusions: A diet rich in phytate increases the potential of intestinal microbiota to degrade phytate. The co-operation of aerobic and anaerobic bacteria is essential for the complete phytate degradation. Significance and Impact of the Study: This study provides insights on the effect of diet on specific metabolic activity of human intestinal microbiota.The work was financed from the funds of the Polish Ministry of Sciences and Higher Education, grant number N N312 434337.Peer Reviewe